6YMQ

TREM2 extracellular domain (19-131) in complex with single-chain variable 4 (scFv-4)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-12
Detector	RAYONIX MX-300
Wavelength(s)	0.9688
Spacegroup name	P 21 21 2
Unit cell lengths	167.510, 180.770, 125.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.990 - 3.070
R-factor	0.2742
Rwork	0.274
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g8r 5ud7
RMSD bond length	0.001
RMSD bond angle	0.392
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER (1.16_3549)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.750	3.180
High resolution limit [Å]	3.070	3.070
Rmeas	0.306
Rpim	0.119
Number of reflections	71788	7077
<I/σ(I)>	6.83
Completeness [%]	99.8	99.9
Redundancy	6.4	6.6
CC(1/2)	0.991	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	39% MPD -- 0.2M ammonium acetate -- 0.1M citrate pH 5.5