6YM9

Mycobacterium tuberculosis FtsZ in complex with GTP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	86
Detector technology	PIXEL
Collection date	2019-07-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	89.063, 89.063, 181.327
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	77.130 - 2.030
R-factor	0.17
Rwork	0.168
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rq7
RMSD bond length	0.012
RMSD bond angle	1.143
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	181.330	2.140
High resolution limit [Å]	2.030	2.030
Number of reflections	51881	7482
<I/σ(I)>	9.3
Completeness [%]	99.0
Redundancy	5.7
CC(1/2)	0.998	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	291.15	27.5% PEG4000, 0.4 M ammonium acetate and 0.1 M Na citrate, soaked with 10 mM GPS for 4 hr.