6YLT

Translation initiation factor 4E in complex with 3-MeBn7GpppG mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	38.300, 38.330, 148.760
Unit cell angles	83.99, 87.78, 76.81

Refinement procedure

Resolution	49.310 - 2.670
R-factor	0.2021
Rwork	0.200
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.008
RMSD bond angle	0.934
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.310	2.766
High resolution limit [Å]	2.670	2.670
Rmerge	0.134	0.616
Rmeas	0.155	0.730
Rpim	0.078	0.383
Number of reflections	23009	2275
<I/σ(I)>	9.76	2.01
Completeness [%]	98.9	96.77
Redundancy	3.8	3.4
CC(1/2)	0.990	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1 M sodium citrate tribasic pH 5.5, 20 % w/v PEG 3350