6YLR

Translation initiation factor 4E in complex with bn7GpppG mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 43
Unit cell lengths	94.047, 94.047, 42.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.060 - 2.195
R-factor	0.226350871249
Rwork	0.224
R-free	0.26955
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.003
RMSD bond angle	0.761
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.060	2.274
High resolution limit [Å]	2.195	2.195
Rmerge	0.147	0.865
Rmeas	0.160	0.935
Rpim	0.060	0.349
Number of reflections	19244	1869
<I/σ(I)>	11.01	1.99
Completeness [%]	99.4	96.92
Redundancy	7	7
CC(1/2)	0.995	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	291	0.1 M Bis-Tris propane pH 9.0, 0.1 M NaCl, 25 % w/v PEG 1500