6YLP

EGFP_in_Acidic_env Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-13
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.5419
Spacegroup name	P 21 21 21
Unit cell lengths	50.970, 62.150, 68.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.120 - 1.550
R-factor	0.1776
Rwork	0.176
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y0g
RMSD bond length	0.012
RMSD bond angle	1.720
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.120	46.120	1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.055	0.029	0.894
Rmeas	0.060	0.031	1.157
Total number of observations	353489
Number of reflections	61255	680	4560
<I/σ(I)>	16.94	65.52	1.03
Completeness [%]	99.9	99.3	99.9
Redundancy	5.771	8.941	2.49
CC(1/2)	0.999	1.000	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	298	PEG 8000, potassium dihydrogen phosphate