6YLN

mTurquoise2 SG P212121 - Directional optical properties of fluorescent proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-03
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.54187
Spacegroup name	P 21 21 21
Unit cell lengths	51.170, 61.270, 68.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.270 - 1.850
R-factor	0.1858
Rwork	0.184
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ztf
RMSD bond length	0.016
RMSD bond angle	1.711
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.270	39.270	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.052	0.023	1.906
Rmeas	0.053	0.024	2.058
Total number of observations	239608
Number of reflections	18997	248	1375
<I/σ(I)>	29.51	128.93	0.95
Completeness [%]	99.9	97.6	99.2
Redundancy	12.613	19.835	6.809
CC(1/2)	1.000	1.000	0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 8000, HEPES, Magnesium chloride