6YK8

OTU-like deubiquitinase from Legionella -Lpg2529

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.070333
Spacegroup name	P 1 21 1
Unit cell lengths	38.534, 140.979, 57.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.530 - 2.420
R-factor	0.2312
Rwork	0.229
R-free	0.28310
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.530	2.506
High resolution limit [Å]	2.420	2.420
Rmerge	0.031	0.377
Number of reflections	23185	2278
<I/σ(I)>	14.85
Completeness [%]	99.2
Redundancy	2
CC(1/2)	0.999	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	25 % PEG3350, 100 mM Tris-HCl pH 8.5, 200 mM NaCl