6YK2

Structure of the AMPA receptor GluA2o ligand-binding domain (S1S2J) in complex with the compound (S)-1-[2'-Amino-2'-carboxyethyl]-5,7-dihydrothieno[3,4-d]pyrimidin- 2,4(1H,3H)-dione at resolution 1.60A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-25
Detector	MARRESEARCH
Wavelength(s)	1.07270
Spacegroup name	P 21 21 2
Unit cell lengths	59.274, 96.530, 48.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.063 - 1.612
R-factor	0.1461
Rwork	0.145
R-free	0.16280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1syh
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	REFMAC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.424	37.424	1.690
High resolution limit [Å]	1.612	5.060	1.612
Rmerge	0.038	0.029	0.107
Rmeas	0.046	0.036	0.134
Total number of observations	99021
Number of reflections	35934	1160	4164
<I/σ(I)>	18.6	21.2	4.9
Completeness [%]	95.8	90	77.2
Redundancy	2.8	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	279	15% PEG4000, 0.3M Lithium Sulfate, 0.1M acetate