6YJR

Crystal structure of unliganded MGAT5 (alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase V) luminal domain.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	59.809, 100.415, 207.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.810 - 2.198
Rwork	0.208
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zic
RMSD bond length	0.009
RMSD bond angle	1.572
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.810	2.490
High resolution limit [Å]	2.198	2.200
Rmerge	0.122	1.223
Rpim	0.047	0.479
Number of reflections	36979	2041
<I/σ(I)>	12.3	1.7
Completeness [%]	93.4
Redundancy	7.8
CC(1/2)	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Citrate pH 5.0 16% w/v PEG6000