6YJD

Lamin A coil2 dimer stabilized by N-terminal capping

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 61 2 2
Unit cell lengths	117.250, 117.250, 93.156
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.620 - 2.900
Rwork	0.253
R-free	0.30420
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.761
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.620	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	8792	847
<I/σ(I)>	20.27	0.99
Completeness [%]	98.3
Redundancy	19.2
CC(1/2)	0.999	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	277	35% (v/v) methanol, 0.2 M MgCl2 and 0.1 M HEPES