6YJ7

DarB fom B. subtilis in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	41.310, 69.260, 105.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.940 - 1.640
R-factor	0.1901
Rwork	0.189
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yav
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.940	50.000	1.710
High resolution limit [Å]	1.640	17.000	1.640
Rmerge	0.028	0.021	0.654
Rmeas	0.029	0.024	0.680
Total number of observations	502479
Number of reflections	37896	46	4316
<I/σ(I)>	40.44	77.34	4.12
Completeness [%]	99.8	86.8	99.8
Redundancy	13.259	5.543	13.526
CC(1/2)	1.000	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1 M Tris/HCl PH 8.5, 32 % w/v polyethylene glycol 4000, 5 % v/v/ glycerol