6YII
Crystal structure of a Class III adenylyl cyclase-like ATP-binding protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.400, 74.680, 149.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.540 - 1.440 |
| Rwork | 0.131 |
| R-free | 0.14730 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (dev 2666) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.540 | 1.490 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.046 | 1.066 |
| Rmeas | 0.050 | |
| Rpim | 0.018 | |
| Number of reflections | 126837 | 12534 |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.3 | |
| CC(1/2) | 1.000 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 22.5 % MPD, 75 mM Na acetate pH 4.8 and 15 mM CaCl2 |
