6YIC
14-3-3 sigma in complex with SMAD4 pS403 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.990, 111.830, 62.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.360 - 1.600 |
| R-factor | 0.1694 |
| Rwork | 0.168 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mhr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.748 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.0.024) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.360 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 38169 | 6243 |
| <I/σ(I)> | 10.86 | 1.91 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.21 | |
| CC(1/2) | 0.995 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M HEPES pH7.5 0.19 M CaCl2 5% glycerol 28% PEG400 |
