6YI0

Human histidine triad nucleotide-binding protein 2 (hHINT2) refined to 1.65 A in P41212 space group

This is a non-PDB format compatible entry.

Replaces:  4NJX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-13
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 41 21 2
Unit cell lengths	76.945, 76.945, 140.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.044 - 1.650
Rwork	0.184
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw2
RMSD bond length	0.009
RMSD bond angle	1.586
Data reduction software	XDS
Data scaling software	Aimless (1.11.21)
Phasing software	MOLREP (11.7.01)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.820	46.820	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.077	0.040	1.082
Rmeas	0.084	0.044	1.191
Rpim	0.035	0.019	0.496
Number of reflections	51576	385	2505
<I/σ(I)>	15.3	36.2	2.1
Completeness [%]	100.0	97.2	100
Redundancy	10.8	7.7	11
CC(1/2)	0.999	0.999	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% (v/v) ethylene glycol, 5% (w/v) PEG 3350, 20 mM MgCl2