6YHV

Structural insights into Pseudomonas aeruginosa Type six secretion system exported effector 8: unliganded Tse8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 1
Unit cell lengths	66.506, 68.078, 77.604
Unit cell angles	72.94, 68.84, 79.27

Refinement procedure

Resolution	42.830 - 1.893
R-factor	0.1873
Rwork	0.186
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6te4
RMSD bond length	0.004
RMSD bond angle	0.573
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.830	1.960
High resolution limit [Å]	1.893	1.893
Rmerge		1.459
Number of reflections	94264	8315
<I/σ(I)>	5.22
Completeness [%]	93.5
Redundancy	3.5
CC(1/2)	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M calcium acetate, 0.1 M Sodium cacodylate pH 6.5 and 18% PEG8,000