6YGQ

EclA N-terminal domain; PllA-like lectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 1 21 1
Unit cell lengths	65.793, 50.461, 73.859
Unit cell angles	90.00, 109.36, 90.00

Refinement procedure

Resolution	40.870 - 1.900
R-factor	0.1829
Rwork	0.181
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ofz
RMSD bond length	0.003
RMSD bond angle	0.621
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.870	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	36259	2350
<I/σ(I)>	8
Completeness [%]	99.9
Redundancy	3.4
CC(1/2)	0.997	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.06 M divalents (magnesium chloride hexahydrate and calcium chloride dihydrate), 0.1 M Tris and bicine, pH 8.5, (40% v/v) glycerol, 20% w/v PEG 4000