6YFV
Crystal structure of Mtr4-Red1 minimal complex from Chaetomium thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2741 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 172.186, 172.186, 145.078 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.706 - 2.750 |
R-factor | 0.2174 |
Rwork | 0.215 |
R-free | 0.25730 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.341 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.710 | 2.848 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.129 | 3.048 |
Number of reflections | 33467 | |
<I/σ(I)> | 25.61 | |
Completeness [%] | 100.0 | |
Redundancy | 39.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 0.1 M MES pH 6.5 30% PEG 300 |