Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 277.471, 396.638, 399.868 |
| Unit cell angles | 69.66, 83.65, 83.49 |
Refinement procedure
| Resolution | 61.150 - 3.500 |
| R-factor | 0.2612 |
| Rwork | 0.261 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | FFT |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 61.150 | 61.150 | 3.690 |
| High resolution limit [Å] | 3.500 | 11.070 | 3.500 |
| Rmerge | 0.038 | 1.481 | |
| Rmeas | 0.434 | 0.054 | 2.095 |
| Rpim | 0.307 | 0.038 | 1.481 |
| Total number of observations | 3182922 | ||
| Number of reflections | 1749831 | 54285 | 262319 |
| <I/σ(I)> | 2.6 | 4.4 | 0.5 |
| Completeness [%] | 87.6 | 86 | 89.9 |
| Redundancy | 1.8 | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 10 % v/v ethanol, 0.1 M Tris-HCl pH 8.5 |
