6YFR

Virus-like particle of bacteriophage NT-391

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	161.917, 162.066, 162.976
Unit cell angles	71.16, 66.67, 66.83

Refinement procedure

Resolution	51.880 - 2.600
R-factor	0.3177
Rwork	0.318
R-free	0.32310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ms2
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	FFT
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.880	51.880	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.105	1.134
Rmeas	0.300	0.130	1.440
Rpim	0.178	0.075	0.875
Total number of observations	690098	29161	71242
Number of reflections	294001	10763	34643
<I/σ(I)>	2.5	7.2	0.5
Completeness [%]	69.7	80.6	56.2
Redundancy	2.3	2.7	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	34% MPD, 0.1 M sodium nitrate, 0.1M sodium acetate pH 4.6