Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 |
| Unit cell lengths | 291.945, 292.531, 469.196 |
| Unit cell angles | 75.79, 77.92, 69.51 |
Refinement procedure
| Resolution | 49.833 - 3.897 |
| R-factor | 0.2821 |
| Rwork | 0.282 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | FFT |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.833 | 49.833 | 4.130 |
| High resolution limit [Å] | 3.897 | 11.420 | 3.900 |
| Rmerge | 0.209 | 0.034 | 1.559 |
| Rmeas | 0.296 | 0.048 | 2.205 |
| Number of reflections | 1227552 | 48206 | 190940 |
| <I/σ(I)> | 2.97 | 16.98 | 0.38 |
| Completeness [%] | 96.2 | 94.9 | 93 |
| Redundancy | 1.8 | ||
| CC(1/2) | 0.985 | 0.997 | 0.151 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 26% MPD, 0.1-0.2 M sodium nitrate, 0.1 M HEPES pH 7.5 |
