6YE8

E.coli's Putrescine receptor PotF complexed with Spermidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	70.830, 70.830, 272.083
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.710 - 1.500
R-factor	0.1621
Rwork	0.162
R-free	0.18260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a99
RMSD bond length	0.009
RMSD bond angle	1.087
Data reduction software	XDS (20180126)
Data scaling software	XDS (20180126)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.710	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.071	2.663
Rmeas	0.075	2.794
Rpim	0.022	0.838
Number of reflections	128236	12611
<I/σ(I)>	17.79	0.76
Completeness [%]	100.0	99.99
Redundancy	11.5	11
CC(1/2)	1.000	0.264

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 10% Jeffamine M600