6YDL

Substrate-free beta-phosphoglucomutase from Lactococcus lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92819
Spacegroup name	P 21 21 21
Unit cell lengths	53.280, 54.070, 81.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.650 - 1.520
R-factor	0.18415
Rwork	0.183
R-free	0.21606
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2whe
Data reduction software	XDS
Data scaling software	xia2 (0.4.0.0)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.650	44.650	1.560
High resolution limit [Å]	1.520	6.800	1.520
Rmerge	0.044	0.017	1.190
Rmeas	0.051	0.020	1.428
Rpim	0.019	0.007	0.561
Number of reflections	36815	486	2658
<I/σ(I)>	18.8	71.6	1.3
Completeness [%]	99.3	98.6	99.3
Redundancy	7.2	6.4	6.3
CC(1/2)	1.000	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	PEG 4000, sodium acetate (200 mM), tris (100 mM), HEPES (50 mM), magnesium chloride (5 mM), EDTA (1 mM), sodium azide (2 mM), beta-phosphoglucomutase (0.6 mM)