6YC5

RT structure of Thaumatin obtained at 1.35 A resolution from crystal grown in a Kapton microchip.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	293.5
Detector technology	PIXEL
Collection date	2017-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	58.390, 58.390, 151.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.480 - 1.350
R-factor	0.1279
Rwork	0.127
R-free	0.14830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1thw
RMSD bond length	0.014
RMSD bond angle	1.314
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.480	1.400
High resolution limit [Å]	1.350	1.350
Number of reflections	52807	3906
<I/σ(I)>	11.4
Completeness [%]	90.1
Redundancy	4.4
CC(1/2)	0.995	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION	7	293.5	20% w/v NaK Tartrate