6YBR

RT structure of Glucose Isomerase obtained at 1.20 A resolution from crystal grown in a Mylar microchip.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	293.5
Detector technology	PIXEL
Collection date	2017-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	93.990, 99.380, 103.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.990 - 1.200
R-factor	0.1215
Rwork	0.121
R-free	0.13050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a8i
RMSD bond length	0.005
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.990	1.240
High resolution limit [Å]	1.200	1.200
Number of reflections	149874	14856
<I/σ(I)>	17
Completeness [%]	100.0
Redundancy	8.86
CC(1/2)	0.994	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION	7	293.5	0.1 M MgCl2, 10% w/v PEG 1K, 0.1 M Hepes pH 7.0