6YAY
Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.060, 78.780, 66.600 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 46.640 - 2.090 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22310 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.498 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17rc2_3619) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 66678 | 10300 |
| <I/σ(I)> | 9.56 | 1.15 |
| Completeness [%] | 98.4 | 94 |
| Redundancy | 3.9 | 3.52 |
| CC(1/2) | 0.998 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | PEG 2000, MES pH6.5, glycerol |
