6YAR
Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of AppCp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.120, 78.430, 65.220 |
Unit cell angles | 90.00, 97.41, 90.00 |
Refinement procedure
Resolution | 45.160 - 1.900 |
R-factor | 0.1798 |
Rwork | 0.178 |
R-free | 0.21760 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.784 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17rc2_3619) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 81355 | 10020 |
<I/σ(I)> | 10.34 | 0.99 |
Completeness [%] | 93.3 | 71 |
Redundancy | 5.16 | 3.6 |
CC(1/2) | 0.999 | 0.652 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | PEG 2000, MES pH6.5, glycerol |