6YA8
Cdc7-Dbf4 bound to ADP-BeF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.150, 61.150, 235.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.860 - 1.790 |
| R-factor | 0.1672 |
| Rwork | 0.166 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f99 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.934 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc4_3812) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.860 | 1.854 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.070 | |
| Number of reflections | 42930 | 4056 |
| <I/σ(I)> | 23.66 | |
| Completeness [%] | 99.0 | 91.15 |
| Redundancy | 11.5 | 11.5 |
| CC(1/2) | 0.999 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.57 | 291.15 | 0.1M MMT pH 6.57, 21% PEG 1500, 15% v/v Acetonitrile, 20mM MgCl2 |
