6Y99
hSTING mutant R232K in complex with 2',3'-cGAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-24 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.918400 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 111.703, 111.703, 35.058 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.493 - 2.984 |
R-factor | 0.2379 |
Rwork | 0.235 |
R-free | 0.28450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ksy |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.493 | 3.091 |
High resolution limit [Å] | 2.984 | 2.984 |
Rmerge | 0.233 | 2.156 |
Rmeas | 0.243 | 2.260 |
Rpim | 0.069 | 0.659 |
Number of reflections | 4861 | 464 |
<I/σ(I)> | 8.57 | 1.1 |
Completeness [%] | 98.7 | |
Redundancy | 12.4 | |
CC(1/2) | 0.988 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M tri-Sodium Citrate dihydrate 20% (w/v) PEG 3350 |