6Y8Y
Structure of Baltic Herring (Clupea Harengus) Phosphoglucomutase 5 (PGM5) with bound Glucose-1-Phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07227 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 47.230, 94.680, 146.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.340 - 1.950 |
R-factor | 0.1703 |
Rwork | 0.168 |
R-free | 0.20970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y8x |
RMSD bond length | 0.004 |
RMSD bond angle | 0.622 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.990 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.116 | 0.816 |
Rmeas | 0.127 | 0.898 |
Rpim | 0.051 | 0.368 |
Number of reflections | 48980 | 3394 |
<I/σ(I)> | 9.2 | 2 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 6.1 | 5.7 |
CC(1/2) | 0.996 | 0.454 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.12M calcium acetate, 0.08 M sodium cacodylate pH 6.5, 14.0% (v/v) PEG 8000, 20% (v/v) glycerol |