6Y7P

The complex between the eight-bladed symmetrical designer protein Tako8 and 1:2 zirconium(IV) Wells-Dawson (ZrWD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	107.347, 72.799, 54.288
Unit cell angles	90.00, 120.38, 90.00

Refinement procedure

Resolution	46.834 - 1.750
R-factor	0.1796
Rwork	0.178
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g6m
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.834	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.088	0.729
Rmeas	0.106	0.863
Rpim	0.058	0.459
Number of reflections	70938	1977
<I/σ(I)>	10.8	2.5
Completeness [%]	99.8
Redundancy	6.4
CC(1/2)	0.996	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M sodium acetate pH 4.5, 3.1M sodium chloride