6Y7O

The complex between the eight-bladed symmetrical designer protein Tako8 and the silicotungstic acid Keggin (STA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97951
Spacegroup name	C 1 2 1
Unit cell lengths	94.554, 133.505, 81.753
Unit cell angles	90.00, 125.27, 90.00

Refinement procedure

Resolution	47.200 - 2.300
R-factor	0.2439
Rwork	0.241
R-free	0.29330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g6m
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.179	1.330
Rmeas	0.201
Rpim	0.090	0.679
Number of reflections	36727	3590
<I/σ(I)>	8.3	2.5
Completeness [%]	100.0
Redundancy	9.8
CC(1/2)	0.989	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	0.1M sodium acetate pH 4.5, 3.1M sodium chloride