6Y7G
Structure of the human RAB3C in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.190, 40.434, 66.362 |
| Unit cell angles | 86.02, 74.16, 66.45 |
Refinement procedure
| Resolution | 63.780 - 2.300 |
| R-factor | 0.2041 |
| Rwork | 0.200 |
| R-free | 0.28950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.498 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.780 | 63.780 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.080 | 0.026 | 0.573 |
| Rmeas | 0.094 | 0.031 | 0.672 |
| Rpim | 0.049 | 0.017 | 0.349 |
| Total number of observations | 57285 | 1045 | 5717 |
| Number of reflections | 16096 | 282 | 1569 |
| <I/σ(I)> | 9.5 | 35.5 | 1.6 |
| Completeness [%] | 98.4 | 98.6 | 97.8 |
| Redundancy | 3.6 | 3.7 | 3.6 |
| CC(1/2) | 0.997 | 0.996 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 25% PEG3350, 0.1M BIS-TRIS pH 6.5 |
