6Y7F
Crystal structure of human ELOVL fatty acid elongase 7 (ELOVL7)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.229, 72.459, 112.043 |
Unit cell angles | 90.00, 100.03, 90.00 |
Refinement procedure
Resolution | 28.680 - 2.052 |
R-factor | 0.2112 |
Rwork | 0.210 |
R-free | 0.22610 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 0.870 |
Data reduction software | DIALS (2.0.3) |
Data scaling software | STARANISO (2.3.25) |
Phasing software | AutoSol (1.17.1-3660) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.262 | 2.182 |
High resolution limit [Å] | 2.052 | 2.052 |
Rmerge | 0.101 | 0.703 |
Rmeas | 0.119 | 0.839 |
Rpim | 0.062 | 0.449 |
Number of reflections | 36724 | 1837 |
<I/σ(I)> | 8.3 | 1.9 |
Completeness [%] | 58.7 | 17.5 |
Redundancy | 6.9 | 6.2 |
CC(1/2) | 0.987 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 279 | 30-36% PEG400 -- 0.1M acetate pH 4.5 -- 0.23M sodium chloride |