6Y7F
Crystal structure of human ELOVL fatty acid elongase 7 (ELOVL7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.229, 72.459, 112.043 |
| Unit cell angles | 90.00, 100.03, 90.00 |
Refinement procedure
| Resolution | 28.680 - 2.052 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.22610 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | DIALS (2.0.3) |
| Data scaling software | STARANISO (2.3.25) |
| Phasing software | AutoSol (1.17.1-3660) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.262 | 2.182 |
| High resolution limit [Å] | 2.052 | 2.052 |
| Rmerge | 0.101 | 0.703 |
| Rmeas | 0.119 | 0.839 |
| Rpim | 0.062 | 0.449 |
| Number of reflections | 36724 | 1837 |
| <I/σ(I)> | 8.3 | 1.9 |
| Completeness [%] | 58.7 | 17.5 |
| Redundancy | 6.9 | 6.2 |
| CC(1/2) | 0.987 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 279 | 30-36% PEG400 -- 0.1M acetate pH 4.5 -- 0.23M sodium chloride |
