6Y76
AP01 - a redesigned transferrin receptor apical domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.122, 119.558, 33.167 |
| Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
| Resolution | 30.700 - 1.980 |
| R-factor | 0.2384 |
| Rwork | 0.234 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Design model |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.822 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc1_3777) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 2.056 |
| High resolution limit [Å] | 1.980 | 1.985 |
| Number of reflections | 15946 | 1375 |
| <I/σ(I)> | 7.61 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.991 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium fluoride and 20% v/v PEG 3350 |
