6Y6P

Structure of Hantaan virus envelope glycoprotein Gn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	53.596, 92.224, 94.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.344 - 1.938
R-factor	0.1687
Rwork	0.167
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5opg
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.344	35.340	1.980
High resolution limit [Å]	1.938	9.090	1.940
Rmerge	0.080	0.040	1.117
Rmeas	0.085	0.044	1.192
Rpim	0.030	0.016	0.411
Total number of observations		2627	18180
Number of reflections	35256	401	2248
<I/σ(I)>	14.6	40.3	1.8
Completeness [%]	99.6	98.2	95.6
Redundancy	8.2	6.6	8.1
CC(1/2)	0.999	0.997	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	25%(w/v) PEG 6000, 0.1M Hepes 7.5, 0.1M LiCl, 20% glycerol