6Y6G
lipopolysaccharide outer core galactosyltransferase WaaB apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 81 |
| Detector technology | CCD |
| Collection date | 2015-02-09 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.823 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.270, 104.270, 88.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.540 - 1.810 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.19560 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.311 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.540 | 1.875 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.125 | 0.863 |
| Number of reflections | 44277 | 3879 |
| <I/σ(I)> | 29 | |
| Completeness [%] | 98.5 | 86.2 |
| Redundancy | 60.1 | 18.2 |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 1% tryptone, 1mM sodium azide, 0.05M HEPES pH7.0 and 20% w/v PEG 3350. |
