6Y6A
Structure of Finch Polyomavirus VP1 in complex with 2-O-Methyl-5-N-acetyl-alpha-D-neuraminic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-06-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99986 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.496, 172.063, 245.616 |
| Unit cell angles | 107.09, 97.93, 93.93 |
Refinement procedure
| Resolution | 49.940 - 2.650 |
| R-factor | 0.2448 |
| Rwork | 0.245 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fmg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.343 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.950 | 2.810 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmeas | 0.271 | 1.130 |
| Number of reflections | 377799 | 13276 |
| <I/σ(I)> | 4.64 | 1.12 |
| Completeness [%] | 94.7 | 96.4 |
| Redundancy | 2.7 | 2.77 |
| CC(1/2) | 0.976 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Magnesium chloride, TRIS, PEG 8000 |
