6Y68

Structure of Maporal virus envelope glycoprotein Gc in postfusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	H 3 2
Unit cell lengths	90.348, 90.348, 506.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.828 - 2.400
R-factor	0.1981
Rwork	0.196
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ljz
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.14rc3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.830	39.830	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.174	0.052	2.752
Rmeas	0.180	0.055	2.843
Rpim	0.046	0.015	0.709
Total number of observations		9035	52803
Number of reflections	31958	697	3310
<I/σ(I)>	11.1	35.5	1
Completeness [%]	100.0	99	100
Redundancy	15.1	13	16
CC(1/2)	0.998	0.999	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	30% (w/v) PEG 400, 0.1M CdCl2, 0.1M Na-acetate 4.6