6Y66
Structure of Goose Hemorrhagic Polyomavirus VP1 in complex with 2-O-Methyl-5-N-acetyl-alpha-D-neuraminic acid
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2017-01-30 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.460, 90.540, 101.220 |
| Unit cell angles | 94.23, 98.09, 107.87 |
Refinement procedure
| Resolution | 49.577 - 1.950 |
| Rwork | 0.165 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fmg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.580 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.147 | 0.761 |
| Number of reflections | 206886 | 20425 |
| <I/σ(I)> | 9.38 | 2.02 |
| Completeness [%] | 97.7 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.993 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Na formate, ammonium acetate, Na citrate tribasic, Na oxamate, Na K tartrate, MES, imidazole, PEG 8000, ethylene glycol |
