Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2017-01-30 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.290, 90.410, 100.840 |
| Unit cell angles | 94.05, 97.90, 108.06 |
Refinement procedure
| Resolution | 45.970 - 1.450 |
| Rwork | 0.148 |
| R-free | 0.17030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fmg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.970 | 1.502 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmeas | 0.068 | 0.702 |
| Number of reflections | 487522 | 46392 |
| <I/σ(I)> | 14.26 | 2.16 |
| Completeness [%] | 95.4 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Na nitrate, Na phosphate dibasic, ammonium sulfate, MES, imidazole, PEG 8000, ethylene glycol |
