6Y62
Crystal structure of the envelope glycoprotein complex of Maporal virus in a prefusion conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.330, 65.013, 144.757 |
Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
Resolution | 35.144 - 2.200 |
R-factor | 0.1958 |
Rwork | 0.194 |
R-free | 0.22620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y5f |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc3_3199) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.070 | 38.070 | 2.260 |
High resolution limit [Å] | 2.200 | 9.330 | 2.200 |
Rmerge | 0.090 | 0.035 | 1.254 |
Rmeas | 0.102 | 0.039 | 1.526 |
Rpim | 0.047 | 0.017 | 0.860 |
Total number of observations | 258945 | 3312 | 13723 |
Number of reflections | 55909 | 743 | 4505 |
<I/σ(I)> | 11 | 33.8 | 0.9 |
Completeness [%] | 99.5 | 95.2 | 98.9 |
Redundancy | 4.6 | 4.5 | 3 |
CC(1/2) | 0.997 | 0.998 | 0.338 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M MgCl2, 0.1M TrisHCl 8.5, 17% (w/v) PEG 20K |