Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2012-04-30 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.07 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.223, 136.416, 85.379 |
Unit cell angles | 90.00, 109.56, 90.00 |
Refinement procedure
Resolution | 44.047 - 1.549 |
Rwork | 0.143 |
R-free | 0.16920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fmg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.548 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.050 | 1.640 |
High resolution limit [Å] | 1.549 | 1.549 |
Rmeas | 0.056 | 0.456 |
Number of reflections | 181696 | 28058 |
<I/σ(I)> | 16.44 | 2.89 |
Completeness [%] | 95.3 | |
Redundancy | 3.8 | |
CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Tacsimate, PEG 3350 |