6Y5P

RING-DTC domain of Deltex1 bound to NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	66.850, 85.254, 130.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.475 - 1.740
R-factor	0.1867
Rwork	0.185
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y5n
RMSD bond length	0.010
RMSD bond angle	1.103
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.850	1.790
High resolution limit [Å]	1.740	1.740
Rmerge	0.056	1.334
Rpim	0.036	0.898
Number of reflections	148052	5662
<I/σ(I)>	16	1
Completeness [%]	100.0	100
Redundancy	6.4	5.9
CC(1/2)	0.999	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	292	0.16M Calcium acetate hydrate, 0.08MSodium cacodylate pH 6.5, 14.4% w/v PEG 8000, 20% v/v Glycerol