6Y5P
RING-DTC domain of Deltex1 bound to NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.850, 85.254, 130.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.475 - 1.740 |
| R-factor | 0.1867 |
| Rwork | 0.185 |
| R-free | 0.21370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6y5n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.103 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.850 | 1.790 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.056 | 1.334 |
| Rpim | 0.036 | 0.898 |
| Number of reflections | 148052 | 5662 |
| <I/σ(I)> | 16 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 5.9 |
| CC(1/2) | 0.999 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 292 | 0.16M Calcium acetate hydrate, 0.08MSodium cacodylate pH 6.5, 14.4% w/v PEG 8000, 20% v/v Glycerol |
