6Y5F

Crystal structure of the envelope glycoprotein prefusion complex of Andes virus - Mutant H953F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97857
Spacegroup name	P 63 2 2
Unit cell lengths	188.505, 188.505, 117.278
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.362 - 3.200
R-factor	0.2141
Rwork	0.211
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ljz 5opg
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.14rc3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.362	49.360	3.420
High resolution limit [Å]	3.200	9.050	3.200
Rmerge	0.197	0.034	2.798
Rmeas	0.204	0.035	2.888
Rpim	0.050	0.009	0.709
Total number of observations		13065	59067
Number of reflections	20744	1016	3657
<I/σ(I)>	13.5	60.7	1.2
Completeness [%]	99.8	98.2	99.4
Redundancy	16.2	12.9	16.2
CC(1/2)	0.999	0.999	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	2.4M (NH4)2,HPO4 0.1M TrisHCl pH=8.5