6Y4K
Crystal structure of human 14-3-3 gamma in complex with CaMKK2 14-3-3 binding motif Ser100 and Fusicoccin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-24 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 225.852, 225.852, 72.963 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.080 - 3.000 |
| R-factor | 0.2616 |
| Rwork | 0.260 |
| R-free | 0.29210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b05 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.589 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.165 | |
| Number of reflections | 22485 | 3562 |
| <I/σ(I)> | 18.91 | 1.01 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 21.83 | 21.01 |
| CC(1/2) | 1.000 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG400, HEPES, magnesium chloride, hexafluoropropanol |
