6Y42
Crystal Structure of RsrR complexed to a 39 basepair DNA fragment of the rsrR promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 197.370, 197.370, 73.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.340 - 4.300 |
R-factor | 0.2684 |
Rwork | 0.267 |
R-free | 0.30020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hsd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.107 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.340 | 4.450 |
High resolution limit [Å] | 4.300 | 4.300 |
Rmerge | 0.087 | 2.130 |
Rmeas | 0.104 | 2.530 |
Rpim | 0.057 | 0.988 |
Number of reflections | 6038 | 3563 |
<I/σ(I)> | 8.8 | 0.9 |
Completeness [%] | 99.5 | |
Redundancy | 5.8 | |
CC(1/2) | 0.995 | 0.360 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | NaCl, MgCl2, CaCl2, PEG1000, PEG3350, MES, anaerobic (in glove box) |