6Y3W

Ternary complex of 14-3-3 sigma (C38N), Estrogen Related Receptor gamma (DBD) phosphopeptide, and disulfide PPI stabilizer 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	82.532, 112.487, 62.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.646 - 1.340
R-factor	0.1643
Rwork	0.164
R-free	0.18660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p1n
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BUSTER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.540	1.360
High resolution limit [Å]	1.340	1.340
Number of reflections	65188	2959
<I/σ(I)>	17.4	2.1
Completeness [%]	99.2
Redundancy	11.4
CC(1/2)	0.999	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.095 M HEPES (pH 7.3), 0.19 M CaCl2, 25% (v/v) PEG 400 and 5% (v/v) glycerol