6Y3J
RING-DTC domains of Deltex 2, bound to ADP-ribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.933, 104.933, 74.544 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.440 - 2.600 |
R-factor | 0.2398 |
Rwork | 0.238 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y22 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.870 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.270 | 1.618 |
Rpim | 0.137 | 1.791 |
Number of reflections | 27953 | 1792 |
<I/σ(I)> | 5.3 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.3 | |
CC(1/2) | 0.994 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 292 | Morpheus condition 39 (Molecular Dimensions) |