6Y3G

Crystal structure of phenylalanine tRNA from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	278
Detector technology	PIXEL
Collection date	2016-09-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 64 2 2
Unit cell lengths	109.618, 109.618, 138.518
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.980 - 3.100
R-factor	0.2163
Rwork	0.215
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yco
RMSD bond length	0.004
RMSD bond angle	0.854
Data reduction software	XDS (1.17.1_3660)
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.300
High resolution limit [Å]	3.100	3.100
Number of reflections	9432	1464
<I/σ(I)>	14.2
Completeness [%]	99.7	98.9
Redundancy	12.5
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	12.4% PEG8000, 0.2M AcCa, 0.1M Hepes pH 7, 0.1M L-Pro