6Y31
NG domain of human SRP54 T117 deletion mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.356, 50.607, 181.629 |
Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
Resolution | 45.724 - 4.001 |
R-factor | 0.3124 |
Rwork | 0.311 |
R-free | 0.33120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y2z |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.724 | 4.140 |
High resolution limit [Å] | 4.000 | 4.000 |
Rpim | 0.213 | 1.250 |
Number of reflections | 13630 | 1343 |
<I/σ(I)> | 2.27 | |
Completeness [%] | 94.6 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.8 M Na2HPO4 , 0.8 M K2HPO4 HEPES 0.1 M (pH 7.5) |